or Paste in a nucleotide sequence:
>sample sequence ggctgccaag aacctgcagg aggcagaaga atggtacaaa tccaagtttg ctgacctctc tgaggctgcc aaccggaaca atgacgccct gcgccaggca aagcaggagt ccactgagta ccggagacag gtgcagtccc tcacctgtga agtggatgcc cttaaaggaa ccaatgagtc cctggaacgc cagatgcgtg aaatggaaga gaactttgcc gttgaagctg ctaactacca agacactatt ggccgcctgc aggatgagat tcagaatatg aaggaggaaa tggctcgtca ccttcgtgaa taccaagacc tgctcaatgt taagatggcc cttgacattg agattgccac ctacaggaag ctgctggaag gcgaggagag caggatttct ctgcctcttc caaacttttc ctccctgaac ctgagggaaa ctaatctgga ttcactccct ctggttgata cccactcaaa aaggacactt ctgattaaga cggttgaaac tagagatgga caggttatca acgaaacttc tcagcatcac gatgaccttg aataaaaatt gcacacactc agtgcagcaa tatattacca

Options:

Functional siRNA selection algorithm by:   Ui-Tei et al., Nucleic Acids Res 32, 936-948 (2004) Link Reynolds et al., Nat Biotechnol 22, 326-330 (2004) Link Amarzguioui et al., BBRC 316, 1050-1058 (2004) Link use combined rule: Ui-Tei + Reynolds + Amarzguioui Ui-Tei + Reynolds × Amarzguioui Ui-Tei × Reynolds × Amarzguioui

Minimization of seed-dependent off-target effects   Seed-duplex stability: Max Tm °C  new!      (for reducing seed-dependent off-target effect)      Ui-Tei et al., Nucleic Acids Res 36, 7100-7109 (2008) Link