Selection of highly functional siRNAs

• Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K. (2004)
  Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference.
  Nucleic Acids Res., 32, 936-948.
• Reynolds A, Leake D, Boese Q, Scaringe S, Marshall WS, Khvorova A. (2004)
  Rational siRNA design for RNA interference.
  Nat. Biotechnol., 22, 326-330.
• Amarzguioui M, Prydz H. (2004)
  An algorithm for selection of functional siRNA sequences.
  Biochem. Biophys. Res. Commun., 316, 1050-1058.

Validation of siRNA selection algorithms

References

• Naito Y, Saigo K, Ui-Tei K. (2008)
  Evaluation of published rational siRNA design algorithms using firefly luciferase gene as a reporter.
  RNA interference research progress, 3-11.